Protein prenylation is a common posttranslational modification that enhances the ability of proteins to interact with membrane components within the cell. In many cases, these prenylated proteins are involved in important human diseases, including aging-related disorders and cancer. To effectively study these proteins or develop therapeutics, large quantities of properly modified proteins are required. Historically, production of fully modified farnesylated and methylated proteins at high yield has been challenging. Recently, an engineered insect cell system which is capable of producing authentically modified KRAS protein was used to generate material for structural studies and assay development. Here we describe protocols for extending this work to other farnesylated and methylated substrates.
CITATION STYLE
Gillette, W., Frank, P., Perkins, S., Drew, M., Grose, C., & Esposito, D. (2019). Production of farnesylated and methylated proteins in an engineered insect cell system. In Methods in Molecular Biology (Vol. 2009, pp. 259–277). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9532-5_20
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