Hepatoprotective activity of flacourtia jangomas (Lour.) raeusch leaves and fruit methanolic extract on paracetamol-induced hepatotoxicity in hepg2 cells

Abstract

Introduction and Aim: Plants have become the current focus of research in treating the various diseases and ailments. Flacourtia jangomas (Lour.) Raeusch belongs to the family Salicaceae. It is a small deciduous fruit tree having immense nutritional and medicinal significance. Different parts of the plant are pharmaceutically used for curing various ailments. In this study, we investigated the hepatoprotective activity of Flacourtia jangomas (Lour.) Raeusch leaves and fruit methanolic extract on Paracetamol induced HepG2 cell line. Methods: The cytotoxic and hepatoprotective properties were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); lipid peroxidation (malondialdehyde (MDA) levels). Results: The increased cell viability of 140.43 ± 4.07% and 133.93 ± 3.20% was observed in HepG2 cells treated with methanolic extract of F. jangomas leaf and fruit extract respectively at 10µg/ml concentration and then decreased along with the rise of F. jangomas leaf and fruit extract concentrations. The level of LDH, ALT, AST and MDA decreased after F. jangomas leaf and fruit treatment compared to negative control. Conclusion: This study suggests that the methanolic Extract of F. jangomas (Lour.) Raeusch leaves (FJL) and fruit (FJF) shows hepatoprotective activity in Paracetamol induced HepG2 cell line by the decrease in AST and ALT activities and LDH and MDA level. Hence, it could be considered as a therapeutic agent in curing liver-related diseases.