High-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy is a nondestructive technique that is used to obtain the metabolite profile of a tissue sample. This method requires minimal sample preparation. However, it is important to handle the sample with care and keep it frozen during preparation to minimize degradation. Here, we describe a typical protocol for HR-MAS analysis of intact tissue. We also include examples of typical pulse sequence programs and quantification methods that are used today.
CITATION STYLE
Giskeødegård, G. F., Cao, M. D., & Bathen, T. F. (2015). High-resolution magic-angle-spinning NMR spectroscopy of intact tissue. Methods in Molecular Biology, 1277, 37–50. https://doi.org/10.1007/978-1-4939-2377-9_4
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