Influence of both glucose and glutamine concentration on mab production rate in chemostat culture of cho cells

Abstract

Chemostat culture for non-adhesive r-CHO at the wide range of glucose and glutamine feed concentration was carried out in the non-serum media. It was shown that the metabolic conditions were changed by the change of residual glucose and residual glutamine concentration. The specific mAb production rate was independent of glucose concentration, but increased in low glutamine concentration. Intracellular fluxes were estimated from extracellular utilization and production rates. It was elucidated that in low glutamine concentration, fluxes of glycolysis and the TCA cycle were accelerated, and the flux of the direct pathway to alpha-ketoglutalate increased with decrease of glucose concentration, however the flux of glutamine decomposition was not influenced by glucose concentration