Experimental design optimization of RP-HPLC method for simultaneous estimation of metsulfuron-methyl, chlorantraniliprole and chlorimuron-ethyl residues in stems of Oryza sativa

  • Adiki S
  • Katakam P
  • Assaleh F
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Abstract

The study aims to develop a chemometrics optimized D-optimal mixture design approach assisted RP- HPLC method for the determination of pesticide residues of metsulfuron-methyl, chlorantraniliprole, and chlorimuron-ethyl in the stems of Oryza sativa. Chromatographic separation was achieved on a C18 column using a mobile phase consisting of a pH 3.5 phosphate buffer and acetonitrile in the ratio 85:15. The optimized HPLC method gave a sharp resolution of metsulfuron-methyl, chlorantraniliprole and chlorimuron-ethyl at a retention time of 2.599 min, 3.805 min and 4.661 min receptively. Linearity was observed in the range 100‒500 µg/mL for metsulfuron-methyl (r2 = 0.999), 4‒20 µg/mL for chlorantraniliprole (r2 = 0.999) and 100‒500 µg/mL for chlorimuron-ethyl (r2 = 0.999). The developed method was validated as per ICH guidelines. The proposed chemometrics optimized RP-HPLC method was found to be successful in the resolution of pesticide residues in the stems of O. sativa. The developed method can be applied to routine quantification of metsulfuron-methyl, chlorantraniliprole and chlorimuron-ethyl.

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Adiki, S. K., Katakam, P., & Assaleh, F. H. (2021). Experimental design optimization of RP-HPLC method for simultaneous estimation of metsulfuron-methyl, chlorantraniliprole and chlorimuron-ethyl residues in stems of Oryza sativa. Future Journal of Pharmaceutical Sciences, 7(1). https://doi.org/10.1186/s43094-021-00372-7

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