Effectiveness of natural and synthetic complexes of porine and O polysaccharide as vaccines against Brucella abortus in mice

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Abstract

A single vaccination of mice with a complex of porin and smooth lipopolysaccharide (porin-S-LPS) extracted virulent Brucella abortus 2308 provided significant protection (P < 0.01 to P < 0.001) against challenge with the same strain, equivalent to that achieved by vaccination with living attenuated B. abortus 19. The porin-S-LPS vaccine given without adjuvant or in several adjuvants (trehalose dimycolate and muramyl dipeptide; the pluronic polymer L-121 and muramyl dipeptide; or complexed with Quil A in immunostimulating complexes) provided equivalent protection. In contrast, one vaccination with porin complexed with rought LPS (porin-R-LPS) from a rough mutant of strain 2308 provided no protection with any adjuvant tested. In one experiment, two inoculations with the porin-R-LPS resulted in a low level of protection, probaby owing to priming of the animals for production of O-polysaccharide-specific antibodies. However, one vaccination with rough-strain porin covalently bound to purified O polysaccharide conferred protection equal to that obtained with natural complexes of porin-S-LPS or with living strain 19. A synthetic vaccine containing long chains of O polysaccharide was more effective than one prepared with short chains. Protective vaccines caused the formation of increased concentrations of circulating O-polysaccharide-specific antibodies, although there were individual exceptions to the quantitative association between O-polysaccharide-specific antibodies and protection. Antibodies specific for porin or R-LPS were found in negligible quantities in vaccinated mice. These results provide additional evidence that the O polysaccharide will constitute an essential component of an effective subcellular vaccine against B. abortus and that O-polysaccharide-specific antibodies play an important role in protective immunity in brucellosis.

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Winter, A. J., Rowe, G. E., Duncan, J. R., Eis, M. J., Widom, J., Ganem, B., & Morein, B. (1988). Effectiveness of natural and synthetic complexes of porine and O polysaccharide as vaccines against Brucella abortus in mice. Infection and Immunity, 56(11), 2808–2817. https://doi.org/10.1128/iai.56.11.2808-2817.1988

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