PERBEDAAN POLA PENGAMBILAN ENZIM LAKTOPEROKSIDASE DENGAN MENGGUNAKAN METODE KROMATOGRAFI

Abstract

The research was carried out to get a method which used as measuring rod of lactoperoxidase (LPO) imobilized, based on reduction of milk fat content by using chromatography and SP Sepharose Fast Flow resin (SP-FF). The study was conducted by using two treatments, a reduction from the fat content before coagulation of casein by rennet (T1), and after coagulation of casein by rennet (T2). The results showed that the T1 produced higher quality than T2. Differences resulting volume of raw materials was possible because of differences in total casein, non-fatsolids, and moisture content in each sample. Differences of LPO immobilization time scales was possible because containt of non-fatsolids in each raw material. High or lowprotein content was influenced by differences in the origin of raw materials LPO medium. While high-low activity LPO influenced the non evenness LPOLPO attached to the resinSP-FF, also was possible because of the extent of sediment total non-fatsolids that can not be separated from raw materials LPO during immobilization.