The Effect of Bromelain from Pineapple (Ananas comosus) on Increasing Protein Digestibility of Milk Replacer for Lamb

Abstract

The research aimed to determine the addition of bromelain for increasing the protein digestibility of milk replacer for lamb. Bromelain extraction from pineapple (Ananas comosus) was precipitated by ammonium sulphate with different concentrations which were 30%, 40%, 50%, 60%, and 70%; then those samples were dialyzed to obtain the best of specific enzyme activities. Milk replacer formulated by mixing skim powder and soybean meal. The research consisted of three replications for each treatment. The treatments were P0 or milk replacer without enzyme (control), P1 or milk replacer with 5% bromelain addition, and P2 or milk replacer with 10% bromelain addition. Specific enzyme activity from each precipitation, dry matter digestibility (DMD), organic matter digestibility (OMD), crude protein digestibility (CPD), and crude fibre digestibility (CFD) analysed by ANOVA according to one way complete randomized design and continued by Duncan multiple range test for significant differences due to treatments. The result showed that bromelain from a 50% saturation level of ammonium sulphate had the best of specific enzyme activity compared to other treatments. The addition by 5% of bromelain enzyme increased DMD, OMD, and CPD (P<0.05). In contrast, the addition of bromelain had no significant effect on CFD compared to the control (P>0.05). To conclude, the addition of purified bromelain in terms of the milk replacer increased the nutrient digestibility of milk replacer.