Generating the fancy protein basket with de novo and combinatorial approaches

Abstract

Under the umbrella of computational techniques, de novo approach occupies a unique role as this method is involved in designing the proteins from scratch. In the first part of the current chapter, we will discuss the principles and applications of the de novo approach along with negative designing technique. In the second part, we will elucidate the combinatorial approach of protein engineering, i.e., a hybrid approach to engineer proteins using both the experimental methods such as directed evolution techniques along with rational and de novo computational techniques. Furthermore, we will discuss various examples that glared the field of protein engineering under combinatorial approach.