MP296PROBIOTICS INCREASE SYSTEMIC REGULATORY T CELL IN CHRONIC KIDNEY DISEASE MICE

Abstract

Introduction and Aims: Emerging evidence showed the important role of kidney-gut crosstalk in diverse pathological processes and alterations in intestinal barrier or dysbiotic gut microbiota have been demonstrated in chronic kidney disease (CKD). Regulatory T cells(Tregs) are known to be important in maintaining immune homeostasis in various inflammatory diseases and recent several in-vitro experiments demonstrated that probiotics could induce an expansion of Tregs. The purpose of this study was to investigate the effect of probiotic treatment on the CKD progression as well as on mesenteric and splenic Tregs. Methods: CKD was induced in 6 week old C56BL/6 mouse by 5/6 nephrectomy (control, n=5, CKD with probiotics, n=5, CKD, n=5). Lactobacillus Rhamnosus R0011 and Lactobacillus Acidophilus R0052 mixture were administered via oral gavage starting 1 day after the operation and continued for 8 weeks following intestinal decontamination. Biochemical, histological analyses as well as immunological analyses were performed at 4 weeks after 5/6 nephrectomy Results: At 8 weeks, Scr increased in CKD group compared to sham operated mice (0.487 ± 0.09 vs 0.24 ± 0.024, p=0.001). Probiotic treatment decreased Scr level (0.377 ± 0.03, p=0.064) and significant increased percentage of splenic Tregs, suggesting that probiotic induced an expansion of Tregs in vivo is partially responsible for the renoprotective effect. Conclusions: Probiotics increase systemic CD4+CD25+ Tregs in CKD mice.