Characterization of a BALB/c Myeloma Library

Abstract

Immunoglobulin-producing myelomas have been classified as shown in Table I. Those producing κ light chain alone have not been included. Of 767 tumors classified, 212 have been saved. Thirty-nine possess antibody activity: anti-5AU, DNP, TNP, purine, AMP, NIP, P-choline, N-acetylglucosamine (NAG), levan, dextran, or Salmonella lipopolysaccharide. With the exception of one IgG3 anti-levan, all proteins were of the IgA class. Other possible antibody activities were looked for in an attempt to test the Jerne hypothesis. Seventy myeloma proteins screened against H-2a, H-2b, H-2k, and H-2d (self) did not react, nor did purified myeloma proteins with antibody activities which have been found more than once. These proteins with repeat antibody activities (anti-levan, anti-dextran, anti-DNP, anti-P-choline, and anti-NAG) were also screened against fetal antigens extracted by the Reisfeld method and showed no direct precipitation or inhibition of precipitation with these antigens.