Fusion of dioxygenase and lignin-binding domains in a novel secreted enzyme from cellulolytic streptomyces sp. SIRexaa-e

Abstract

Background: Fusions of dioxygenase and CBMs have been predicted in cellulolytic microbes. Results: SACTE-2871 is unique two-domain enzyme that reacts with caffeoyl-CoA and shows preferential binding to synthetic lignins. Conclusion: SACTE-2871 is an intradiol dioxygenase that is targeted to growing surfaces of lignin. Significance: SACTE-2871 can destroy precursors needed by the plant for de novo lignin biosynthesis as part of its natural wounding response. © 2013 by The American Society.