Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.
CITATION STYLE
Külzer, S., Bittl, V., & Przyborski, J. M. (2015). Fractionation of Plasmodium-infected human red blood cells to study protein trafficking. Methods in Molecular Biology (Clifton, N.J.), 1270, 71–80. https://doi.org/10.1007/978-1-4939-2309-0_5
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