Retinal neurotransmitter interaction as reflected in horizontal cell spatial behaviour.

Abstract

The effects of 5-hydroxytryptamine (5-HT) and its precursors 5-hydroxytryptophan (5-HTP) and L-tryptophan (L-Tryp) on the spatial properties of horizontal cells were studied in the isolated and perfused retina of the teleost Eugerres plumieri. All three compounds produce a contraction of the receptive field, evaluated by the ratio of responses evoked by local and distant light stimuli. This is the result of cell uncoupling, revealed by the hindrance to diffusion of intracellularly injected Lucifer yellow. Similar effects are produced by dopamine (DA) and the effectiveness is DA much greater than 5-HT greater than 5-HTP greater than L-Tryp. All these effects are blocked by Haloperidol. HPLC studies of endogenous DA release reveal that it occurs when isolated retinas are incubated with 50 mM potassium, 10 microM 5-HT or 5-HTP, but is not found with up to 1 mM L-Tryp. The results indicate that indolaminergic cells induce the release of DA from interplexiform cells, which in turn uncouples horizontal cells in the fish retina.