Dendritic cells (DCs) detect and respond to microbes or their components by producing cytokines and other molecules that can activate the proliferation and differentiation pathways of T cells. Investigation of DC responses to pathogens would thus provide important insights into how T-cell responses most appropriate for the pathogen are induced. Here, we describe methods for the use of mixed leukocyte reactions, to determine the proliferative and cytokine responses of murine splenic T cells in response to co-culture with bone marrow-derived DCs stimulated with Candida albicans.
CITATION STYLE
Gibson, J., Gow, N. A. R., & Wong, S. Y. C. (2012). Murine bone marrow-derived dendritic cells and t-cell activation by candida albicans. In Methods in Molecular Biology (Vol. 845, pp. 261–275). Humana Press Inc. https://doi.org/10.1007/978-1-61779-539-8_17
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