CaCl2 enhanced somatic embryogenesis in manihot esculenta crantz

Abstract

Primary cassava somatic embryos were induced on a medium without CaCl 2, however, no or only a few secondary somatic embryos were formed from them. With 15mM CaCl2 in the medium for induction of cassava primary embryos, more secondary somatic embryos were produced from them, and they were much effective in maintaining their embryogenic capacity than the controls of embryos which were induced without CaCl2.