Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.
CITATION STYLE
Tormos-Pérez, M., Pérez-Hidalgo, L., & Moreno, S. (2016). Fission yeast cell cycle synchronization methods. In Methods in Molecular Biology (Vol. 1369, pp. 293–308). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3145-3_20
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