Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines

  • Ili C
  • Brebi P
  • Garcia P
  • et al.
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Abstract

© 2015, Universidad de la Frontera. All rights reserved. Overexpression of Short and Raji variants of Cellular FLICE-like inhibitory protein (c-FLIP) is capable of inhibiting apoptosis, while the function of the Long isoform depends of c-FLIPL concentration in cells. The aim of this study was to determine the effects of c-FLIPL knockdown in cervical cell lines. SiHa, C-4I and C-33A cervical cancer cell lines were analyzed. c-FLIPL level expression was determined by quantitative real-time PCR and western blotting. c-FLIPL was transiently downregulated by siRNA. The effects of knockdown of c-FLIPL on cell viability, proliferation and apoptosis were assessed by comparing with scrambled siRNA-transfected cells. SiHa and C-4I c-FLIPL knockdown cells showed increased viability compared with scrambled siRNA-transfected cells (P < 0.05), while C-33A cells did not show significant differences. Ki-67 and PCNA immunocytochemistry was performed to evaluate proliferation on these cervical cancer cell lines. SiHa cells with c-FLIPL knockdown showed elevated expression of Ki-67 protein compared with their scrambled counterparts (P < 0.0001), while C-33A c-FLIPL knockdown cells showed a significantly lower in PCNA expression (P < 0.01) compared with control. All three c-FLIP-transfected cell lines showed a higher level of apoptosis compared with their scrambled controls. Our results suggest that c-FLIPL could have effects in proliferation and apoptosis in cervical cancer cell lines.

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APA

Ili, C. G., Brebi, P., Garcia, P., Leal, P., Lopez, J., Tapia, O., … Roa, J. C. (2015). Effects of c-FLIPL Knockdown in Cervical Uterine Carcinoma Cell Lines. International Journal of Morphology, 33(2), 638–646. https://doi.org/10.4067/s0717-95022015000200036

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