Improvement in Wound Healing by Epidermal Growth Factor (EGF) Ointment. II. Effect of Protease Inhibitor, Nafamostat, on Stabilization and Efficacy of EGF in Burn

29Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

The healing effect of human epidermal growth factor (hEGF) on second degree burn was studied in rats. No improvement in wound healing was found on topical application of EGF alone to burn sites, but an ointment containing EGF and nafamostat mesilate (NM), a protease inhibitor, accelerated the healing rate of burns. The dry weight of the granulation tissue on the wound site in the group treated with EGF plus NM ointment did not change, although that in other groups decreased. After treatment with EGF ointment containing NM, the content of uronic acid, as an index of acid mucopolysaccharide, at 3 d after burn rapidly increased and had recovered to nearly normal levels at 7 d after burn. However, the uronic acid content in the other groups (control, EGF alone, and NM alone) showed a higher value at 7 d than at 3 d. When compared with the control values significant increases in hydroxyproline, as an index of collagen, in the wound site were observed at 7 d after treatment with EGF ointment containing NM. The degradation of [125I]EGF in burned tissue homogenate decreased significantly in a concentration-dependent manner in the presence of NM. Body weights did not change after treatment with EGF plus NM ointment, although the body weights of other treatment groups decreased after burn, suggesting that EGF ointment containing the protease inhibitor, NM, alleviated the effects of burn shock. These findings indicate that the stabilization of EGF at the wound site is an important factor for the expression of its healing effects. © 1991, The Pharmaceutical Society of Japan. All rights reserved.

References Powered by Scopus

New synthetic inhibitors of C1r̄, C1 esterase, thrombin, plasmin, kallikrein and trypsin

309Citations
N/AReaders
Get full text

Enhancement of epidermal regeneration by biosynthetic epidermal growth factor

271Citations
N/AReaders
Get full text

Pharmacological Studies of FUT-175, Nafamstat Mesilate I. Inhibition of Protease Activity in in Vitro and in Vivo Experiments

246Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Enhanced keratinocyte proliferation and migration in co-culture with fibroblasts

96Citations
N/AReaders
Get full text

Adipose tissue extract enhances skin wound healing

72Citations
N/AReaders
Get full text

Pentadecapeptide BPC 157 cream improves burn-wound healing and attenuates burn-gastric lesions in mice

70Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kiyohara, Y., Komada, F., Iwakawa, S., Hirai, M., Fuwa, T., & Okumura, K. (1991). Improvement in Wound Healing by Epidermal Growth Factor (EGF) Ointment. II. Effect of Protease Inhibitor, Nafamostat, on Stabilization and Efficacy of EGF in Burn. Journal of Pharmacobio-Dynamics, 14(1), 47–52. https://doi.org/10.1248/bpb1978.14.47

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

75%

Professor / Associate Prof. 1

13%

Lecturer / Post doc 1

13%

Readers' Discipline

Tooltip

Medicine and Dentistry 4

50%

Biochemistry, Genetics and Molecular Bi... 2

25%

Materials Science 1

13%

Pharmacology, Toxicology and Pharmaceut... 1

13%

Save time finding and organizing research with Mendeley

Sign up for free