Distinct lymphocyte subpopulations display discrete metabolic profiles and are differently affected by metabolic resource variations, making the analysis of lymphocyte survival in a complex tissue in response to metabolic stress highly challenging. Here we describe a flow cytometry-based method allowing simultaneous cell identification and viable cell counting in mixed lymphocyte populations without extensive cell subset purification procedures. The example provided herein illustrates the role of AMPK in T lymphocyte survival in response to the mitochondrial poison oligomycin.
CITATION STYLE
Denanglaire, S., Pirnay, T., Leo, O., & Andris, F. (2018). A flow cytometry-based protocol to measure lymphocyte viability upon metabolic stress. In Methods in Molecular Biology (Vol. 1732, pp. 465–476). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7598-3_29
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