BIOCHEMICAL CHARACTERIZATION AND IDENTIFICATION OF PROBIOTIC Lactobacillus FOR SWINE

Abstract

The aim of the present work was to select lactic acid bacteria from the gastrointestinal tract of pigs and establihs their biochemical characterization. Strains P01-001 and P08-002 were selected out of a total pf 33 bile resistant strains (5 por cent) of bile because they presented the best survival rateat room temperature and after freeze-drying. Complementary studies on aspects like the ability to grow at different temepratures and resistance to inhibitory substances such as bile 10 per cent, phenol 0,4 per cent, NaCl 4 per cent and 8 percent were performed. The selected strains P01-001 and P08-002 were identified biochemically through the API 50 test as Lactobacillus and classified as heterofermentative bacteria by gas production from glucose and by the presence of lactic and acetic acids in Man, Rogosa and Sharpe (MRS) supematants detected by HPLC. The strains P01-001 and P08-002 growing at 45ºC and tolerat to 10 per cent bile, 0,4 per cent phenol and 4 per cent NaCl will be studied in animal trials as potential probiotic agents