Immunoblotting using radiolabeled reagents for detection.

Abstract

Development of immunoblots is commonly performed using enzyme-labeled antibodies, which convert soluble substrates into insoluble colored products. A simple, rapid, and sensitive alternative method that produces low background and allows a rapid quantitative evaluation is the use of radiolabeled antibodies or protein A conjugates. Here, we describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification.