Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry

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Abstract

The vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability. © 2007 The Institute of Brewing & Distilling.

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Novak, J., Basarova, G., Teixeira, J. A., & Vicente, A. A. (2007). Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry. Journal of the Institute of Brewing, 113(3), 249–255. https://doi.org/10.1002/j.2050-0416.2007.tb00284.x

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