An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle

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Abstract

Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle. © 2011 American Association of Veterinary Laboratory Diagnosticians.

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APA

Wilkowsky, S. E., Bareiro, G. G., Mon, M. L., Moore, D. P., Caspe, G., Campero, C., … Romano, M. I. (2011). An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle. Journal of Veterinary Diagnostic Investigation, 23(5), 971–976. https://doi.org/10.1177/1040638711416845

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