Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing "minigene" in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest.
CITATION STYLE
Orjalo, A. V. Jr., & Johansson, H. E. (2016). Stellaris ® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature ... Long Non-Coding RNAs: Methods and Protocols, 1402(January), 119–134. Retrieved from http://link.springer.com/10.1007/978-1-4939-3378-5%0Ahttp://www.ncbi.nlm.nih.gov/pubmed/26721495%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4770877%0Ahttp://link.springer.com/10.1007/978-1-4939-3378-5_18
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