Toxoplasma gondii rhoptry protein ROP16 mediates partially SH-SY5Y cells apoptosis and cell cycle arrest by directing ser15/37 phosphorylation of p53

21Citations
Citations of this article
39Readers
Mendeley users who have this article in their library.

Abstract

Toxoplasma rhoptries, an unusual set of apical organelles that are associated with Toxoplasma infection may cause subversion of the host cell functions. Parasite rhoptry protein 16 (ROP16) is a regulator of host cell transcription during cell invasion in which it migrates into the host cell cytoplasm and subsequently localizes to the nucleus. In the present study, we found that overexpression of ROP16 could partially mediate human neuroblastoma SH-SY5Y apoptosis (12.47%) and cell cycle arrest in G1 phase (60.77%) in a p53 dependent manner by influencing the expression of Bax/Bcl-2 and p21/CDKs. ROP16 was identified to co-localize with p53, a novel direct interaction partner in the nucleus of SH-SY5Y. Furthermore, SH-SY5Y apoptosis via the mitochondria-dependent p53 pathway and cell cycle arrest caused by ROP16 dealt with direct serine 15/37 phosphorylation of p53. Our studies provide a new mechanism by which ROP16 interacts with the nucleus proteins which subsequently subverts the host cells functions.

References Powered by Scopus

Modes of p53 Regulation

1393Citations
N/AReaders
Get full text

p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by ser-46-phosphorylated p53

1051Citations
N/AReaders
Get full text

Control of mitochondrial apoptosis by the Bcl-2 family

790Citations
N/AReaders
Get full text

Cited by Powered by Scopus

SILAC-based proteomic analysis reveals that salidroside antagonizes cobalt chloride-induced hypoxic effects by restoring the tricarboxylic acid cycle in cardiomyocytes

40Citations
N/AReaders
Get full text

Strategies developed by toxoplasma gondii to survive in the host

35Citations
N/AReaders
Get full text

Eimeria tenella ROP kinase EtROP1 induces G0/G1 cell cycle arrest and inhibits host cell apoptosis

26Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Chang, S., Shan, X., Li, X., Fan, W., Zhang, S. Q., Zhang, J., … Mao, Z. (2015). Toxoplasma gondii rhoptry protein ROP16 mediates partially SH-SY5Y cells apoptosis and cell cycle arrest by directing ser15/37 phosphorylation of p53. International Journal of Biological Sciences, 11(10), 1215–1225. https://doi.org/10.7150/ijbs.10516

Readers over time

‘15‘16‘17‘18‘19‘20‘21‘22‘23‘24036912

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 26

81%

Professor / Associate Prof. 4

13%

Lecturer / Post doc 1

3%

Researcher 1

3%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 10

38%

Immunology and Microbiology 8

31%

Biochemistry, Genetics and Molecular Bi... 7

27%

Pharmacology, Toxicology and Pharmaceut... 1

4%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1
Social Media
Shares, Likes & Comments: 1

Save time finding and organizing research with Mendeley

Sign up for free
0