Stem Cell Bioprinting: Functional 3D Neural Mini‐Tissues from Printed Gel‐Based Bioink and Human Neural Stem Cells (Adv. Healthcare Mater. 12/2016)

Abstract

On page 1429 G. G. Wallace, J. M. Crook, and co-workers report the first example of fabricating neural tissue by 3D bioprinting human neural stem cells. A novel polysaccharide based bioink preserves stem cell viability and function within the printed construct, enabling self-renewal and differentiation to neurons and supporting neuroglia. Neurons are predominantly GABAergic, establish networks, are spontaneously active, and show a bicuculline induced increased calcium response.