Effet du nombre de spermatozoïdes sur la fertilité de la semence conservée 24 heures chez le lapin

Abstract

Effect of conservation time and number of rabbit spermatozoa on fertility. The effect of conservation time and number of rabbit spermatozoa on fertility and prolificity were deter- mined. In the first assay, the fertility of spermatozoa conserved 0!4 h, 10-14 h and 26-30 h from semen pools at 12 and 24 millions per mL (6 and 12 millions by insemination dose) was evaluated on lac- tating multiparous does. A dose of 12 millions of spermatozoa refrigerated at 16-18 °C for 26-30 h showed a similar fertility rate than 6 millions of spermatozoa at 0! h (80.6 °lo and 82.7 %, respec- tively). In the second assay, seminal parameters between spermatozoa fresh and refrigerated, and fer- tility and prolificity rate according to the reproductive status of does were evaluated. No differences were observed in the seminal parameters (normal acrosome rate: 92 %, motility rate: 75 %, curvilinear rate-VCL-: 41 1 pm.s!’ , linear index: 57 %), fertility at birth (76 %) and prolificity (9.6 alive born) between fresh and refrigerated semen.