In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Cozzi, J., Anegon, I., Braun, V., Gross, A. C., Merrouche, C., & Cherifi, Y. (2009). Pronuclear DNA injection for the production of transgenic rats. Methods in Molecular Biology, 561, 73–88. https://doi.org/10.1007/978-1-60327-019-9_5
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