Transcriptome-based analysis of mitogen-activated protein kinase cascades in the rice response to Xanthomonas oryzae infection

Abstract

Background: Mitogen-activated protein (MAP) kinase cascades, with each cascade consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK), play important roles in dicot plant responses to pathogen infection. However, no single MAP kinase cascade has been identified in rice, and the functions of MAP kinase cascades in rice − pathogen interactions are unknown. Results: To explore the contribution of MAP kinase cascade in rice in response to Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight, one of the devastating diseases of rice worldwide, we performed a comprehensive expression analysis of rice MAP kinase cascade genes. We transcriptionally analyzed all the 74 MAPKKK genes, 8 MAPKK, and 17 MAPK genes in two pairs of susceptible and resistant rice lines, with each pair having the same genetic background, to determine the rice response to Xoo infection. The expression of a large number of MAP kinase cascade genes changed in response to infection, and some of the genes also showed different expression in resistant and susceptible reactions. In addition, some MAPKKK genes co-expressed with MAPKK and/or MAPK genes, and MAPKK genes co-expressed with MAPK genes. Conclusions: These results provide a new perspective regarding the putative roles of rice MAP kinase gene candicates and potential cascade targets for further characterization in rice–pathogen interactions.