Measurement of Cytoplasmic Calcium Activity with Ion-Selective Microelectrodes

  • Sanders D
  • Miller A
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Abstract

Several different techniques are available for measurement of cytoplasmic calcium activity (or its logarithmic equivalent, pCac). Optical probes involve monitoring changes in one of a number of signals: dye absorbance, fluorescence intensity or bioluminescence. A conceptually different approach stems from the use of Ca2+-selective electrodes in which the sensor is embedded in a liquid membrane plugging the tip of a micropipette.

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Sanders, D., & Miller, A. J. (1986). Measurement of Cytoplasmic Calcium Activity with Ion-Selective Microelectrodes. In Molecular and Cellular Aspects of Calcium in Plant Development (pp. 149–156). Springer US. https://doi.org/10.1007/978-1-4613-2177-4_19

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