Cervical cancer is one of the most common gynecologic malignancies and poses a serious health problem worldwide. Identification and characterization of cervical cancer stem cells may facilitate the development of novel strategies for the treatment of advanced and metastatic cervical cancer. Breast cancer-resistance protein (Bcrp1)-positive cells were selected from a population of parent HeLa cells using flow cytometry. The invasion capacity of Bcrp1 - positive and -negative cells was analyzed with a Boyden chamber invasion test. The tumorigenicity of these cells was determined by in vivo transplantation in non-obesity diabetes/severe combined immunodeficiency (NOD/SCID) mice. The Bcrp1-positive subpopulation accounted for about 7% of the parent HeLa cell population. The proliferative capacity of the Bcrp1-positive cells was greater than that of the Bcrp1-negative cells (P < 0.05). In the invasion assay, the Bcrp1-positive cells demonstrated a greater invasive capacity through the artificial basement membrane than their Bcrp1-negative counterparts. Following transplantation of 10 4 cells, only the Bcrp1-positive cells formed tumors in NOD/SCID mice. When 10 5 or 10 6 cells were transplanted, the tumor incidence and the tumor mass were greater in the Bcrp1-positive groups than those in the Bcrp1-negative groups (P < 0.05). The Bcrp1-positive subpopulation cervical cancer stem cells. © Springer Science+Business Media B.V. 2011.
CITATION STYLE
Zhang, S. L., Wang, Y. S., Zhou, T., Yu, X. W., Wei, Z. T., & Li, Y. L. (2012). Isolation and characterization of cancer stem cells from cervical cancer HeLa cells. Cytotechnology, 64(4), 477–484. https://doi.org/10.1007/s10616-012-9436-3
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