Non-photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A new in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin-dependent alteration in the chlorophyll environment. © 2000 Federation of European Biochemical Societies.
Wentworth, M., Ruban, A. V., & Horton, P. (2000). Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin. FEBS Letters, 471(1), 71–74. https://doi.org/10.1016/S0014-5793(00)01369-7