One challenge in facing the application of biomedical Raman spectroscopy is that the Raman signal is acquired in a dark operation room. It is inconvenient for both the operator and the patient because it is difficult for the operator to accurately and precisely locate the target in the dark environment, and the patient feels uncomfortable in such a setting. In this note, we propose a method to implement biomedical Raman measurement with an illumination source, by multiple filtering of the illumination and the collection optics. Experimental results are demonstrated on skin Raman measurement under 785-nm excitation.
Zhao, J., Short, M., Braun, T., Lui, H., McLean, D., & Zeng, H. (2014). Clinical Raman measurements under special ambient lighting illumination. Journal of Biomedical Optics, 19(11), 111609. https://doi.org/10.1117/1.jbo.19.11.111609