Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus

Abstract

A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84T, was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8- 1.2×3.5-5.0μm), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84T produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 °C and pH 6.5. The major cellular fatty acids were iso-C15: 0, iso-C15: 0 3-OH, iso-C17: 0 and C16: 0, distinguishing strain CL-84T from closely related species. The DNA G+C content of strain CL-84T was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84T shared 90-95% similarity with characterized genera within the phylum Synergistetes, family Synergistaceae. Phylogenetic analysis showed that strain CL-84T was related to, but distinct from, Cloacibacillus evryensis. Based on these findings, we propose that strain CL-84T represents a novel species of the genus Cloacibacillus.We further propose the name Cloacibacillus porcorum sp. nov. be designated for this species. The type strain is CL-84T (=DSM 25858T=CCUG 62631T). An emended description of the genus Cloacibacillus is provided.