Conditional expression of MCM7 increases tumor growth without altering DNA replication activity

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Abstract

The minichromosome maintenance (MCM) 2-7 complex is a putative DNA helicase complex that facilitates the initiation of DNA replication. Here, we generated a cell line MCM7+/-/MCM7-FLAG, in which one allele of MCM7 is mutated whereas a tetracycline-repressible promoter could manipulate the expression of exogenous MCM7 protein. Overexpressed MCM7 protein supports efficient DNA replication of Epstein-Barr virus oriP and rapid formation of tumors in nude mice without altering the activity of cellular DNA replication. This system provides a unique setting for studying the function of MCM7 and for screening for potential therapeutics for malignant tumors. © 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Yoshida, K., & Inoue, I. (2003). Conditional expression of MCM7 increases tumor growth without altering DNA replication activity. FEBS Letters, 553(1–2), 213–217. https://doi.org/10.1016/S0014-5793(03)01018-4

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