Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis

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Abstract

A system for controlled targeting of heterologous protein was developed in the food-grade bacterium Lactococcus lactis. It is composed of the L. lactis strain NZ9000 and of two broad host range expression vectors pCYT:Nuc and pSEC:Nuc for, respectively, cytoplasmic and secreted staphylococcal nuclease (Nuc) nisin-inducible production. The level of intracellular production of Nuc measured with pCYT:Nuc (3 mg l-1) is significantly lower than the one obtained with pSEC:Nuc (∼20 mg l-1). The secretion efficiency (SE) of Nuc is estimated to be ∼70%, corresponding to ∼15 mg of secreted Nuc l-1. Furthermore, we established that Nuc production continued in L. lactis 10 h after a 1-h nisin-pulse induction. This system was then used for intra- and extracellular production of a protein of therapeutical interest in L. lactis, the ovine interferon-omega (IFN-ω). The SE and the quantity of secreted active IFN-ω were evaluated respectively to be ∼70% and ∼1 mg l-1 (∼two-fold higher than the cytoplasmic form). © 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Bermúdez-Humarán, L. G., Langella, P., Commissaire, J., Gilbert, S., Le Loir, Y., L’Haridon, R., & Corthier, G. (2003). Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis. FEMS Microbiology Letters, 224(2), 307–313. https://doi.org/10.1016/S0378-1097(03)00475-0

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