// Yeo Song Lee 1, * , So-Young Kim 1, * , Su Jeong Song 1 , Hye Kyung Hong 1 , Yura Lee 1 , Bo Young Oh 2 , Woo Yong Lee 2, 3 , Yong Beom Cho 2, 3, 4 1 Samsung Biomedical Research Institute, Sungkyunkwan University, Seoul, Republic of Korea 2 Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea 3 Department of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul, Republic of Korea 4 Department of Medical Device Management & Research, SAIHST, Sungkyunkwan University, Seoul, Republic of Korea * These authors have contributed equally to this work Correspondence to: Yong Beom Cho, e-mail: email@example.com Keywords: CCL7, CCR3, ERK-JNK signaling, metastasis, colon cancer Received: August 24, 2015     Accepted: April 22, 2016     Published: May 6, 2016 ABSTRACT Chemokine ligand 7 (CCL7) enhances cancer progression and metastasis via epithelial-mesenchymal transition (EMT). However, little is known about the molecular mechanism of CCL7-induced EMT signaling cascade in colon cancer. Thus, the objective of this study was to investigate CCL7-induced EMT signaling pathway and its role in the progression and metastasis of colon cancer. To demonstrate the effect of CCL7 on EMT induction, HCT116 and HT29 cells overexpressing CCL7 were generated. CCL7-induced EMT and its downstream signaling pathway were evaluated by both in vitro and in vivo experiments. In in vitro studies, CCL7 was found to interplay with CC chemokine receptor 3 (CCR3), resulting in enhanced cellular proliferation, invasion, and migration via ERK and JNK signaling pathway. To validate these findings, we established ectopic and orthotopic mouse models injected with CCL7-overexpressed cells. In ectopic mouse models, we observed that CCL7-overexpressed cells grew significantly faster than control cells. In orthotopic mouse models, we found that liver and lung metastasis developed only in mice injected with CCL7-overexpressed cells. This study is the first one focusing on the EMT cascade via CCL7-CCR3-ERK-JNK signaling axis in colon cancer. Our novel findings will improve our understanding on the mechanism of metastatic process and provide potential therapeutic strategies for preventing metastasis in colon cancer.
Lee, Y. S., Kim, S.-Y., Song, S. J., Hong, H. K., Lee, Y., Oh, B. Y., … Cho, Y. B. (2016). Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways. Oncotarget, 7(24). https://doi.org/10.18632/oncotarget.9209