Measuring respiration rates in marine fish larvae: Challenges and advances

Abstract

Metabolic costs can be extremely high in marine fish larvae and gaining reliable estimates of the effects of intrinsic and extrinsic factors on those costs is important to understand environmental constraints on early growth and survival. This review provides an historical perspective of measurements of larval marine fish respiration (O2 consumption) including the methods (Winkler, manometric, polarographic, paramagnetic and optodes) and systems (closed system to intermittent-flow) used. This study compares and systematically reviews the results (metabolic rates, ontogenetic changes and taxonomic differences) obtained from 59 studies examining 53 species from 30 families. Standard (anaesthetized or darkness), routine and active respiration rates were reported in 14, 94 and 8% of the studies and much more work has been performed on larvae of temperate (88%) compared with tropical (9%) and polar (3%) species. More than 35% of the studies have been published since 2000 owing to both advances in oxygen sensors and the growing emphasis on understanding physiological effects of environmental change. Common protocols are needed to facilitate cross-taxa comparisons such as the effect of temperature (Q10: 1·47-3·47), body mass (slope of allometric changes in O2 consumption rate from 0·5 to 1·3) and activity level on metabolic costs as measured via respiration rate. A set of recommendations is provided that will make it easier for researchers to design measurement systems, to judge the reliability of measurements and to make inter-comparisons among studies and species.