Metabolic Pathway Analysis of Saccharomyces Cerevisiae

Abstract

In metabolic engineering, analysis of pathways is central and it involves quantification of pathway fluxes and how these fluxes are controlled. Therefore metabolic pathway analysis rely on metabolic control analysis and metabolic flux analysis. This paper introduces the basic concepts of metabolic pathway analysis that serves as a valuable analytical tool for examination of cellular metabolism especially in connection with designing directed genetic changes to achieve specific objectives, e.g. increase flux toward a product of interest. In the paper, metabolic pathway analysis of the glycolysis and the galactose metabolism of Saccharomyces cerevisiae are used to illustrate the power of this analytical tool.