Leptospires, the etiological agents of leptospirosis, are fastidious slow-growing organisms. Here we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.
CITATION STYLE
Goarant, C., Girault, D., Thibeaux, R., & Soupé-Gilbert, M. E. (2020). Isolation and Culture of Leptospira from Clinical and Environmental Samples. In Methods in Molecular Biology (Vol. 2134, pp. 1–9). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0459-5_1
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