Determination of sensitivity limit in quantative analysis of polycyclic aromatic hydrocarbons by Gc-ms

Abstract

The limit of detection (LOD) and the limit of quantitation (LOQ) for 16 individual PAHs from the Environmental Protection Agency (EPA) list (naphthalene, acenaphthylene, acenaphtene, fluorene, phenanthrene antrachene, fluoranthene, pyrene, benzo(a)anthracene, chrysene benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h) anthracene, benzo(g,h,i)perylene and indeno(1,2,3-cd)pyrene) were determined by Gas Chromatography ? Mass Spectrometry (GC-MS). Determination was performed by SCAN (digital linear scanning) and SIM (selected ion monitoring) techniques preceded by the optimization of the experimental factors. The limit of detection obtained by the SCAN technique was in the range of 0.179 ? 15.236 ng, and the quantitation limit in the range of 1.017 ? 50.788 ng. SIM technique enabled LOD and LOQ in the range of 0.036 ? 13.886 pg and 0.119 ? 46.287 pg respectively.U radu je definisana granica detekcije (LOD) i granica kvantifikacije (LOQ) pri odredjivanju sadrzaja 16 pojedinacnih policiklicnih aromaticnih ugljovodonika (PAHs) (naftalen, acenaftilen, acenaften, fluoren, fenantren antracen, fluoranten, piren, benzo(a)antracen, krizen, benzo(b)fluoranten benzo(k)fluoranten, benzo(a)piren, dibenzo(a,h)antracen, benzo(g,h,i)perilen i indeno(1,2,3-cd)piren) primenom gasne hromatografije i masene spektrometrije. Odredjivanje je izvodjeno SCAN i SIM tehnikom uz prethodnu optimizaciju uslova odredjivanja. Granica detekcije ostvarena SCAN tehnikom kretala se u intervalu 0,179 ? 15,236 ng, a granica kvantifikacije 1,017 ? 50,788 ng, dok je primenom SIM tehnike ostvarena garnica detekcije u intervalu 0,036 ? 13, 886 pg, a granica kvantifikacije 0,119 ? 46,267 pg.