The generation of xenograft models, which support the growth of human tissue in animals, forms an important part of a researcher's tool kit and enhances the ability to understand the initiation and development of cancer in vivo. Especially in the context of the brain tumor-initiating cell (BTIC), a xenograft model allows for careful characterization of BTIC roles in tumor initiation, growth, and relapse. Here, we detail a set of procedures which describe the isolation, enrichment, and intracranial injection of human BTICs from patient samples to generate xenograft models of a human brain tumor.
CITATION STYLE
Qazi, M., Mann, A., Van Ommeren, R., Venugopal, C., McFarlane, N., Vora, P., & Singh, S. K. (2014). Generation of murine xenograft models of brain tumors from primary human tissue for in vivo analysis of the brain tumor-initiating cell. Methods in Molecular Biology, 1210, 37–49. https://doi.org/10.1007/978-1-4939-1435-7_4
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