Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the γ-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins were purified from serum using protein L-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the γ-globulins (r = 0.2-0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test.
CITATION STYLE
Vanderschaeghe, D., Debruyne, E., Van Vlierberghe, H., Callewaert, N., & Delanghe, J. (2013). CE Analysis of γ-Globulin Mobility and Potential Clinical Utility (pp. 249–257). https://doi.org/10.1007/978-1-62703-029-8_22
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