Effective use of high CO2 efflux at the soil surface in a tropical understory plant

Abstract

Many terrestrial plants are C3 plants that evolved in the Mesozoic Era when atmospheric CO2 concentrations ([CO2]) were high. Given current conditions, C3 plants can no longer benefit from high ambient [CO2]. Kaempferia marginata Carey is a unique understory ginger plant in the tropical dry forests of Thailand. The plant has two large flat leaves that spread on the soil surface. We found a large difference in [CO2] between the partly closed space between the soil surface and the leaves (638 μmol mol-1) and the atmosphere at 20 cm above ground level (412 μmol mol-1). This finding indicates that the plants capture CO2 efflux from the soil. Almost all of the stomata are located on the abaxial leaf surface. When ambient air [CO2] was experimentally increased from 400 to 600 μmol mol-1, net photosynthetic rates increased by 45 to 48% under near light-saturated conditions. No significant increase was observed under low light conditions. These data demonstrate that the unique leaf structure enhances carbon gain by trapping soil CO2 efflux at stomatal sites under relatively high light conditions, suggesting that ambient air [CO2] can serve as an important selective agent for terrestrial C3 plants.