The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution. © Springer Science+Business Media New York 2013.
CITATION STYLE
Noh, H., Barnthip, N., Parhi, P., & Vogler, E. A. (2013). Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. Methods in Molecular Biology, 1025, 157–166. https://doi.org/10.1007/978-1-62703-462-3_12
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