Circulating Extracellular Vesicles Impair Mesenchymal Stromal Cell Differentiation Favoring Adipogenic Rather than Osteogenic Differentiation in Adolescents with Obesity

3Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

Excess body weight has been considered beneficial to bone health because of its anabolic effect on bone formation; however, this results in a poor quality bone structure. In this context, we evaluated the involvement of circulating extracellular vesicles in the impairment of the bone phenotype associated with obesity. Circulating extracellular vesicles were collected from the plasma of participants with normal weight, as well as overweight and obese participants, quantified by flow cytometry analysis and used to treat mesenchymal stromal cells and osteoblasts to assess their effect on cell differentiation and activity. Children with obesity had the highest amount of circulating extracellular vesicles compared to controls. The treatment of mesenchymal stromal cells with extracellular vesicles from obese participants led to an adipogenic differentiation in comparison to vesicles from controls. Mature osteoblasts treated with extracellular vesicles from obese participants showed a reduction in differentiation markers in comparison to controls. Children with obesity who regularly performed physical exercise had a lower circulating extracellular vesicle amount in comparison to those with a sedentary lifestyle. This pilot study demonstrates how the high amount of circulating extracellular vesicles in children with obesity affects the bone phenotype and that physical activity can partially rescue this phenotype.

References Powered by Scopus

Insulin sensitivity indices obtained from oral glucose tolerance testing: Comparison with the euglycemic insulin clamp

4816Citations
N/AReaders
Get full text

Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis

2167Citations
N/AReaders
Get full text

Challenges and directions in studying cell–cell communication by extracellular vesicles

625Citations
N/AReaders
Get full text

Cited by Powered by Scopus

From Metabolic Syndrome to Type 2 Diabetes in Youth

7Citations
N/AReaders
Get full text

Converging protective pathways: Exploring the linkage between physical exercise, extracellular vesicles and oxidative stress

5Citations
N/AReaders
Get full text

Macrophage-derived extracellular vesicles regulate skeletal stem/progenitor Cell lineage fate and bone deterioration in obesity

2Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Peruzzi, B., Urciuoli, E., Mariani, M., Chioma, L., Tomao, L., Montano, I., … Manco, M. (2023). Circulating Extracellular Vesicles Impair Mesenchymal Stromal Cell Differentiation Favoring Adipogenic Rather than Osteogenic Differentiation in Adolescents with Obesity. International Journal of Molecular Sciences, 24(1). https://doi.org/10.3390/ijms24010447

Readers' Seniority

Tooltip

Lecturer / Post doc 2

40%

PhD / Post grad / Masters / Doc 2

40%

Researcher 1

20%

Readers' Discipline

Tooltip

Nursing and Health Professions 2

50%

Biochemistry, Genetics and Molecular Bi... 1

25%

Psychology 1

25%

Article Metrics

Tooltip
Mentions
News Mentions: 1
Social Media
Shares, Likes & Comments: 33

Save time finding and organizing research with Mendeley

Sign up for free