Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for the random introduction of these codons into open reading frames resulting in protein libraries that are scanned with unnatural amino acid residues. These libraries can enable large-scale mutagenesis experiments aimed at understanding and improving protein function.
CITATION STYLE
Liu, J., & Cropp, T. A. (2012). Experimental methods for scanning unnatural amino acid mutagenesis. Methods in Molecular Biology, 794, 187–197. https://doi.org/10.1007/978-1-61779-331-8_11
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