Abstract
The scientific and engineering aspects of design, development, scale-up, and manufacture of monoclonal antibodies are summarized in this chapter by outlining the key elements in the development of the expression cell line, cell culture, cell harvest, and protein purification process and exploring the effect of process technologies on production economics.
Author supplied keywords
- Bioreactor
- Bioreactor sterility
- Cell culture scale up
- Cell harvest
- Cell line screening
- Chinese hamster ovary cell line
- Continuous centrifugation
- Cost modelling
- Cost of goods
- Depth filtration
- Disc stack centrifuge
- Economies of scale
- Genetic stability
- Host cell protein
- Ion exchange
- Medium optimization
- Phenotypic stability
- Process economics
- Protein expression
- Run rate
- Sigma factor chromatography
- Viral clearance
Cite
CITATION STYLE
APA
Ram, K., Hatton, D., Ahuja, S., Bender, J., Hunter, A., & Turner, R. (2017). Protein production in eukaryotic cells. In Topics in Medicinal Chemistry (Vol. 21). Springer Verlag. https://doi.org/10.1007/7355_2016_3
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