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Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter

Eukaryotic Cell (2011) 10(12) 1724-1732
DOI: 10.1128/EC.05199-11
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Abstract

High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of 13NO -2, giving average K m and V max values of 48±8 μM and 228±49 nmol mg -1 dry weight (DW) h -1, respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r 2=0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagines residues N122 (transmembrane domain 3 [Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed. © 2011, American Society for Microbiology. All Rights Reserved.

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APA

Unkles, S. E., Symington, V. F., Kotur, Z., Wang, Y., Yaeesh Siddiqi, M., Kinghorn, J. R., & Glass, A. D. M. (2011). Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter. Eukaryotic Cell, 10(12), 1724–1732. https://doi.org/10.1128/EC.05199-11

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